Close Menu
  • Business
    • Invest
    • Real Estate
    • Services
  • Architecture
    • Decorating
    • Design
    • Home Improvement
    • Interiors
    • Make it Modern
  • Fashion
    • Clothes
    • New Look
    • Street Fashion
    • Style Hunter
    • Vogue
  • Sports
    • Cricket
    • Football
    • Racing

Subscribe to Updates

Get the latest creative news from FooBar about art, design and business.

What's Hot

5 Reasons to Choose Concrete Flooring

December 6, 2023

Smart Strategies For Small Businesses To Succeed In The Market

November 27, 2023

Get Your Appliances Back on Track with GoTech and John H.: Expert Appliance Repair in Edmonton!

November 23, 2023
Facebook X (Twitter) Instagram
  • Technology
  • Digital Marketing & SEO
Publician
  • Business
    • Invest
    • Real Estate
    • Services
  • Architecture
    • Decorating
    • Design
    • Home Improvement
    • Interiors
    • Make it Modern
  • Fashion
    • Clothes
    • New Look
    • Street Fashion
    • Style Hunter
    • Vogue
  • Sports
    • Cricket
    • Football
    • Racing
Publician
Home » CRO Processes: The Guide To Setting Up An Accurate Luminex ELISA Assay
Health & Fitness

CRO Processes: The Guide To Setting Up An Accurate Luminex ELISA Assay

Sean CollinsBy Sean CollinsJune 8, 2022No Comments3 Mins Read
Facebook Twitter Pinterest LinkedIn Telegram Tumblr Email
Share
Facebook Twitter LinkedIn Pinterest Email

Luminex assays are magnetic bead-based immunoassays employing similar principles to sandwich ELISA assays. Luminex assays are multiplex assays that can simultaneously analyze up to 100 analytes with less sample volume compared to traditional ELISAs. They employ color-coded beads dyed with different amounts of red and infrared fluorophores. As Luminex ELISAs can quantify multiple analytes, Luminex multiplex cytokine assays and Luminex protein assays have become crucial in understanding biological and disease processes.

Although Luminex assays are similar to sandwich ELISAs, they have unique requirements. In the current article, we share a complete guide to setting up an accurate Luminex ELISA assay for several clinical drug development studies.

Working and Principle of Luminex ELISA assay

First, researchers mix the study sample with color-coded beads that are coated with capture antibodies specific to the analyte of interest. Once the antibodies bind to the analytes, biotinylated detection antibodies are added to form an antibody-antigen sandwich. Later phycoerythrin-conjugated streptavidin reporter molecules are added that bind to the detection antibodies.

Magnetic beads are read on dual-laser flow-based systems such as FlexMap analyzer and Luminex 200. One laser in the dual-laser system classifies and determines the analyte, whereas the second laser deduces the magnitude of the PE-streptavidin signal. This generated signal is directly proportional to the amount of bound analyte. Other than flow-based analyzers, researchers can also use Luminex MAGPIX analyzers. This analyzer uses a magnet to capture and hold the beads while illuminating them through two different LEDs. One LED detects the analyte, while the other determines the magnitude of the PE-streptavidin signal.

Sample preparation

Sample preparation is crucial for Luminex ELISA assays. Depending on each drug development pathway, there may be different samples for analysis. However, Luminex ELISAs need a minimum 2-fold dilution. Besides, highly abundant biomarkers may need additional dilutions. Although Luminex supports several biological samples, they are not suitable for analyzing icteric, lipemic, and hemolyzed samples. Below we share sample preparation for a range of biological matrices.

  • Plasma: Use heparin or EDTA as an anticoagulant while collecting plasma. It is necessary to centrifuge the plasma within 30 mins of collection, for 15 mins at 1000*g.
  • Cell culture supernates: Centrifuge the samples to remove particulates.
  • Tissue Lysate: Rinse the tissue, cut it into smaller pieces and homogenize it in PBS. Add cell lysis buffer to lyse at room temperature. Finally, centrifuge to remove debris.
  • Serum: Allow the sample to clot at room temperature. Centrifuge for 15 mins at 1000*g and remove the serum.
  • Saliva: Centrifuge the saliva for 5 mins at 10,000*g and collect the aqueous section for analysis.
  • Urine: Collect the first-morning urine (mid-stream) into a sterile container. Remove particulate matter by centrifuging for 4 mins at 16,000*g.
  • Human milk: Analyze the sample immediately or store them below -20℃.
  • Platelet-poor plasma: Use heparin or EDTA to collect plasma and centrifuge at 1000*g for 15 mins within 30 mins of collection. Additional centrifugation for 10 mins at 10000*g is recommended to remove the platelets.

Hence, Luminex assays are ideal for several biological matrices. However, it is necessary to note that Luminex assays have not been validated against all study samples, and researchers should verify them before using any specific Luminex kit.

Share. Facebook Twitter Pinterest LinkedIn Tumblr Email
Sean Collins
  • Website

population journal words speaks iphone 9 price in Pakistan Samsung Galaxy A32 price in Pakistan Samsung Galaxy S25 Ultra price in Pakistan Infinix Note 12 Turbo Vs Xiaomi Redmi Note 10 Pro 5G price in Pakistan patrick clancy

Related Posts

Comprehensive Guide: Who Should Avoid Taking CBD?

October 24, 2023

CBD Bud: Exploring the Benefits and Uses of this Natural Alternative

October 9, 2023

Sports and Mental Health: The Powerful Impact on Athletes’ Well-being

September 8, 2023

Achieve Your Health Goals with a Women’s Health Magazine Subscription

February 28, 2023

Top 3 10 LPM Oxygen Concentrators- Price and Features

January 11, 2023

Hyaluronic acid: How to use it, benefits, side effects and more

January 10, 2023

Comments are closed.

Demo
Top Posts

5 Reasons to Choose Concrete Flooring

December 6, 2023

Why It’s Smart to Use Authentication Apps for Multifactor Security

January 11, 2020

Save $90 on The HS700E 4K Drone, An Ideal Beginner

January 12, 2020
Don't Miss

5 Reasons to Choose Concrete Flooring

December 6, 2023

People are quick to dismiss concrete flooring as cold and unwelcoming. Others relegate concrete flooring…

Smart Strategies For Small Businesses To Succeed In The Market

November 27, 2023

Get Your Appliances Back on Track with GoTech and John H.: Expert Appliance Repair in Edmonton!

November 23, 2023

The Best Bingo Variations to Play Online

November 23, 2023
Stay In Touch
  • Facebook
  • YouTube
  • TikTok
  • WhatsApp
  • Twitter
  • Instagram
Latest Reviews
8.9

Review: Dell’s New Tablet PC Can Survive -20f And Drops

January 15, 2021

Review: Kia EV6 2022 The Best Electric Vehicle Ever?

January 14, 2021
72

Review: Animation Software Business Share, Market Size and Growth

January 14, 2021
Demo

  • Home
All rights are reserved

Type above and press Enter to search. Press Esc to cancel.